Construct: shRNA TRCN0000270240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001085414.2-371s21c1
DNA Barcode:
GAACCTACAAGTGCTTGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pramef6 (195555)
Gene Description:
PRAME family member 6
Transcript:
RefSeq NM_001085414.2 (CURRENT)
Match location:
Position 371 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCTACAAGTGCTTGATTT
Hairpin Sequence:
5'-CCGG-GAACCTACAAGTGCTTGATTT-CTCGAG-AAATCAAGCACTTGTAGGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCTACAAGTGCTTGATTTCTCGAGAAATCAAGCACTTGTAGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCTACAAGTGCTTGATTTCTCGAGAAATCAAGCACTTGTAGGTTC-3'

Other clones with same target sequence:

(none)