Construct: shRNA TRCN0000270248

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001101452.1-159s21c1
DNA Barcode:
ACAGATCCTCTGCTTAATTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sult3a2 (215895)
Gene Description:
sulfotransferase family 3A, member 2
Transcript:
RefSeq NM_001101452.1 (CURRENT)
Match location:
Position 159 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270248)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGATCCTCTGCTTAATTTG
Hairpin Sequence:
5'-CCGG-ACAGATCCTCTGCTTAATTTG-CTCGAG-CAAATTAAGCAGAGGATCTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGATCCTCTGCTTAATTTGCTCGAGCAAATTAAGCAGAGGATCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGATCCTCTGCTTAATTTGCTCGAGCAAATTAAGCAGAGGATCTGT-3'

Other clones with same target sequence:

(none)