Construct: shRNA TRCN0000270402

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024709.3-1008s21c1
DNA Barcode:
TGCCTTTCCACTGGCTATTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krtap10-10 (544710)
Gene Description:
keratin associated protein 10-10
Transcript:
RefSeq NM_001024709.3 (CURRENT)
Match location:
Position 1008 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270402)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCTTTCCACTGGCTATTTG
Hairpin Sequence:
5'-CCGG-TGCCTTTCCACTGGCTATTTG-CTCGAG-CAAATAGCCAGTGGAAAGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCTTTCCACTGGCTATTTGCTCGAGCAAATAGCCAGTGGAAAGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCTTTCCACTGGCTATTTGCTCGAGCAAATAGCCAGTGGAAAGGCA-3'

Other clones with same target sequence:

(none)