Construct: shRNA TRCN0000270450
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001024709.3-950s21c1
- DNA Barcode:
- GCTTCACGTGGTATGAGTTAC
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Krtap10-10 (544710)
- Gene Description:
- keratin associated protein 10-10
- Transcript:
- RefSeq NM_001024709.3 (CURRENT)
- Match location:
- Position 950 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000270450)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTTCACGTGGTATGAGTTAC
- Hairpin Sequence:
-
5'-CCGG-GCTTCACGTGGTATGAGTTAC-CTCGAG-GTAACTCATACCACGTGAAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTTCACGTGGTATGAGTTACCTCGAGGTAACTCATACCACGTGAAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTTCACGTGGTATGAGTTACCTCGAGGTAACTCATACCACGTGAAGC-3'
Other clones with same target sequence:
(none)
