Construct: shRNA TRCN0000271207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018883.2-781s21c1
DNA Barcode:
ACCACGTGAATGTAGTCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Camkk1 (55984)
Gene Description:
calcium/calmodulin-dependent protein kinase kinase 1, alpha
Transcript:
RefSeq NM_018883.2 (CURRENT)
Match location:
Position 781 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCACGTGAATGTAGTCAAAT
Hairpin Sequence:
5'-CCGG-ACCACGTGAATGTAGTCAAAT-CTCGAG-ATTTGACTACATTCACGTGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCACGTGAATGTAGTCAAATCTCGAGATTTGACTACATTCACGTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCACGTGAATGTAGTCAAATCTCGAGATTTGACTACATTCACGTGGT-3'

Other clones with same target sequence:

(none)