Construct: shRNA TRCN0000272159

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001103153.1-652s21c1
DNA Barcode:
ATGGAGAGCTCCAGGGTATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm10334 (100040233)
Gene Description:
predicted gene 10334
Transcript:
RefSeq NM_001103153.1 (CURRENT)
Match location:
Position 652 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272159)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGAGAGCTCCAGGGTATAG
Hairpin Sequence:
5'-CCGG-ATGGAGAGCTCCAGGGTATAG-CTCGAG-CTATACCCTGGAGCTCTCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGAGAGCTCCAGGGTATAGCTCGAGCTATACCCTGGAGCTCTCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGAGAGCTCCAGGGTATAGCTCGAGCTATACCCTGGAGCTCTCCAT-3'

Other clones with same target sequence:

(none)