Construct: shRNA TRCN0000273148

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004938.2-2763s21c1
DNA Barcode:
CGACATCCAGAACGCTTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAPK1 (1612)
Gene Description:
death associated protein kinase 1
Transcript:
RefSeq NM_004938.2 (NON-CURRENT)
Match location:
Position 2763 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273148)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACATCCAGAACGCTTATTT
Hairpin Sequence:
5'-CCGG-CGACATCCAGAACGCTTATTT-CTCGAG-AAATAAGCGTTCTGGATGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACATCCAGAACGCTTATTTCTCGAGAAATAAGCGTTCTGGATGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACATCCAGAACGCTTATTTCTCGAGAAATAAGCGTTCTGGATGTCG-3'

Other clones with same target sequence:

  1. TRCN0000000985