Construct: shRNA TRCN0000000985
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_004938.x-2727s1c1
- DNA Barcode:
- CGACATCCAGAACGCTTATTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DAPK1 (1612)
- Gene Description:
- death associated protein kinase 1
- Transcript:
- RefSeq NM_004938.1 (NON-CURRENT)
- Match location:
- Position 2727 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000000985)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGACATCCAGAACGCTTATTT
- Hairpin Sequence:
-
5'-CCGG-CGACATCCAGAACGCTTATTT-CTCGAG-AAATAAGCGTTCTGGATGTCG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGACATCCAGAACGCTTATTTCTCGAGAAATAAGCGTTCTGGATGTCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGACATCCAGAACGCTTATTTCTCGAGAAATAAGCGTTCTGGATGTCG-3'
Other clones with same target sequence:
-
TRCN0000273148
