Construct: shRNA TRCN0000273739

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019757.1-652s21c1
DNA Barcode:
GATCTCTAAGATTCCCTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fzr1 (56371)
Gene Description:
fizzy/cell division cycle 20 related 1 (Drosophila)
Transcript:
RefSeq NM_019757.1 (CURRENT)
Match location:
Position 652 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273739)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCTCTAAGATTCCCTTCAA
Hairpin Sequence:
5'-CCGG-GATCTCTAAGATTCCCTTCAA-CTCGAG-TTGAAGGGAATCTTAGAGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCTCTAAGATTCCCTTCAACTCGAGTTGAAGGGAATCTTAGAGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCTCTAAGATTCCCTTCAACTCGAGTTGAAGGGAATCTTAGAGATC-3'

Other clones with same target sequence:

  1. TRCN0000027682