Construct: shRNA TRCN0000274495

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007396.4-1288s21c1
DNA Barcode:
GCCCAGTTGCTCAATGAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acvr2a (11480)
Gene Description:
activin receptor IIA
Transcript:
RefSeq NM_007396.4 (CURRENT)
Match location:
Position 1288 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274495)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGTTGCTCAATGAATAT
Hairpin Sequence:
5'-CCGG-GCCCAGTTGCTCAATGAATAT-CTCGAG-ATATTCATTGAGCAACTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGTTGCTCAATGAATATCTCGAGATATTCATTGAGCAACTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGTTGCTCAATGAATATCTCGAGATATTCATTGAGCAACTGGGC-3'

Other clones with same target sequence:

  1. TRCN0000022659