Transcript: Mouse NM_007396.4

Mus musculus activin receptor IIA (Acvr2a), mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Acvr2a (11480)
Length:
5681
CDS:
664..2205

Additional Resources:

NCBI RefSeq record:
NM_007396.4
NBCI Gene record:
Acvr2a (11480)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148374 ATTGCAGAAACCATGGCTAG pXPR_003 AGG 890 58% 7 -0.1215 Acvr2a ACVR2A 77931
Download CSV

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_007396.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000022661 GCTAGAGGATTGGCATATTTA pLKO.1 1552 CDS 100% 15.000 21.000 N Acvr2a n/a
2 TRCN0000274494 GCTAGAGGATTGGCATATTTA pLKO_005 1552 CDS 100% 15.000 21.000 N Acvr2a n/a
3 TRCN0000285238 GGTGTTGGAGGGTGCTATAAA pLKO_005 1770 CDS 100% 15.000 12.000 N Acvr2a n/a
4 TRCN0000274492 GACCTGGCTAATCAAGTATTT pLKO_005 2493 3UTR 100% 13.200 10.560 N Acvr2a n/a
5 TRCN0000244976 GGCTAGAGGATTGGCATATTT pLKO_005 1551 CDS 100% 15.000 10.500 N ACVR2A n/a
6 TRCN0000000556 CAGGAAGTTGTTGTGCATAAA pLKO.1 1948 CDS 100% 13.200 9.240 N ACVR2A n/a
7 TRCN0000022659 GCCCAGTTGCTCAATGAATAT pLKO.1 1288 CDS 100% 13.200 9.240 N Acvr2a n/a
8 TRCN0000274495 GCCCAGTTGCTCAATGAATAT pLKO_005 1288 CDS 100% 13.200 9.240 N Acvr2a n/a
9 TRCN0000022663 GAGGGCAATATGTGTAATGAA pLKO.1 979 CDS 100% 5.625 3.938 N Acvr2a n/a
10 TRCN0000022662 CCTCCCAAAGAATCTAGTCTA pLKO.1 2182 CDS 100% 4.950 3.465 N Acvr2a n/a
11 TRCN0000000552 CCTTAAATGAACTACTGCTAT pLKO.1 2560 3UTR 100% 4.950 3.465 N ACVR2A n/a
12 TRCN0000000554 GCTAATGTGGTCTCTTGGAAT pLKO.1 1504 CDS 100% 4.950 3.465 N ACVR2A n/a
13 TRCN0000022660 CCTGTGGCTAATCACAGCATT pLKO.1 1449 CDS 100% 0.495 0.347 N Acvr2a n/a
14 TRCN0000274493 CCTGTGGCTAATCACAGCATT pLKO_005 1449 CDS 100% 0.495 0.347 N Acvr2a n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_007396.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05768 pDONR223 100% 94.5% 99.6% None (many diffs) n/a
2 ccsbBroad304_05768 pLX_304 0% 94.5% 99.6% V5 (many diffs) n/a
3 TRCN0000476792 GCATCAATGGAAATCAAAGACCTG pLX_317 33.3% 94.5% 99.6% V5 (many diffs) n/a
4 ccsbBroadEn_14529 pDONR223 0% 94.5% 99.6% None (many diffs) n/a
5 ccsbBroad304_14529 pLX_304 0% 94.5% 99.6% V5 (many diffs) n/a
6 TRCN0000469988 CAAGCGACGGGCTTTCGGACTAAA pLX_317 25.7% 94.5% 99.6% V5 (many diffs) n/a
7 TRCN0000489398 TCACAGGTGCGAGCCCAGATTTCA pLX_317 21.5% 94.5% 99.6% V5 (not translated due to prior stop codon) (many diffs) n/a
8 TRCN0000489291 AGTTCGTTACTAGGGTGTGACGGC pLX_317 23.3% 94.4% 99.6% V5 (many diffs) n/a
Download CSV