Construct: shRNA TRCN0000274794

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026551.3-589s21c1
DNA Barcode:
AGTGTACTGTGACCGTGATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dcakd (68087)
Gene Description:
dephospho-CoA kinase domain containing
Transcript:
RefSeq NM_026551.3 (CURRENT)
Match location:
Position 589 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274794)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGTACTGTGACCGTGATAC
Hairpin Sequence:
5'-CCGG-AGTGTACTGTGACCGTGATAC-CTCGAG-GTATCACGGTCACAGTACACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGTACTGTGACCGTGATACCTCGAGGTATCACGGTCACAGTACACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGTACTGTGACCGTGATACCTCGAGGTATCACGGTCACAGTACACT-3'

Other clones with same target sequence:

(none)