Construct: shRNA TRCN0000275177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017528.2-137s21c1
DNA Barcode:
GTTCGCAACTCACGGATGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BUD23 (114049)
Gene Description:
BUD23 rRNA methyltransferase and ribosome maturation factor
Transcript:
RefSeq NM_017528.2 (NON-CURRENT)
Match location:
Position 137 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCGCAACTCACGGATGATT
Hairpin Sequence:
5'-CCGG-GTTCGCAACTCACGGATGATT-CTCGAG-AATCATCCGTGAGTTGCGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCGCAACTCACGGATGATTCTCGAGAATCATCCGTGAGTTGCGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCGCAACTCACGGATGATTCTCGAGAATCATCCGTGAGTTGCGAAC-3'

Other clones with same target sequence:

  1. TRCN0000139958