Construct: shRNA TRCN0000276484

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001418.3-2325s21c1
DNA Barcode:
CCCTTTGGTGAAATCCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF4G2 (1982)
Gene Description:
eukaryotic translation initiation factor 4 gamma 2
Transcript:
RefSeq NM_001418.3 (NON-CURRENT)
Match location:
Position 2325 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276484)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTTGGTGAAATCCTATTT
Hairpin Sequence:
5'-CCGG-CCCTTTGGTGAAATCCTATTT-CTCGAG-AAATAGGATTTCACCAAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTTGGTGAAATCCTATTTCTCGAGAAATAGGATTTCACCAAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTTGGTGAAATCCTATTTCTCGAGAAATAGGATTTCACCAAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000150297