Construct: shRNA TRCN0000276491

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022756.4-104s21c1
DNA Barcode:
GGCGGAAACATTGGCAAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MEAF6 (64769)
Gene Description:
MYST/Esa1 associated factor 6
Transcript:
RefSeq NM_022756.4 (NON-CURRENT)
Match location:
Position 104 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276491)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCGGAAACATTGGCAAATTT
Hairpin Sequence:
5'-CCGG-GGCGGAAACATTGGCAAATTT-CTCGAG-AAATTTGCCAATGTTTCCGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCGGAAACATTGGCAAATTTCTCGAGAAATTTGCCAATGTTTCCGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCGGAAACATTGGCAAATTTCTCGAGAAATTTGCCAATGTTTCCGCC-3'

Other clones with same target sequence:

(none)