Construct: shRNA TRCN0000277108

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153125.2-3723s21c1
DNA Barcode:
ACTTGGCATCCTACTACTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec16a (227648)
Gene Description:
SEC16 homolog A, endoplasmic reticulum export factor
Transcript:
RefSeq NM_153125.2 (CURRENT)
Match location:
Position 3723 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277108)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTGGCATCCTACTACTATT
Hairpin Sequence:
5'-CCGG-ACTTGGCATCCTACTACTATT-CTCGAG-AATAGTAGTAGGATGCCAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTGGCATCCTACTACTATTCTCGAGAATAGTAGTAGGATGCCAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTGGCATCCTACTACTATTCTCGAGAATAGTAGTAGGATGCCAAGT-3'

Other clones with same target sequence:

(none)