Construct: shRNA TRCN0000277395

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025347.2-522s21c1
DNA Barcode:
CAACCACATGATCAAAGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ypel3 (66090)
Gene Description:
yippee-like 3 (Drosophila)
Transcript:
RefSeq NM_025347.2 (CURRENT)
Match location:
Position 522 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277395)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCACATGATCAAAGACAA
Hairpin Sequence:
5'-CCGG-CAACCACATGATCAAAGACAA-CTCGAG-TTGTCTTTGATCATGTGGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCACATGATCAAAGACAACTCGAGTTGTCTTTGATCATGTGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCACATGATCAAAGACAACTCGAGTTGTCTTTGATCATGTGGTTG-3'

Other clones with same target sequence:

  1. TRCN0000191128