Transcript: Mouse NM_025347.2

Mus musculus yippee-like 3 (Drosophila) (Ypel3), mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Ypel3 (66090)
Length:
957
CDS:
196..555

Additional Resources:

NCBI RefSeq record:
NM_025347.2
NBCI Gene record:
Ypel3 (66090)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025347.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000277394 ATGACTGTCACCGGAGGTATA pLKO_005 239 CDS 100% 10.800 8.640 N Ypel3 n/a
2 TRCN0000243056 GAACTCAACCACATGATCAAA pLKO_005 517 CDS 100% 5.625 4.500 N YPEL3 n/a
3 TRCN0000243055 AGAGCAGCCAGAAGTACAAAG pLKO_005 479 CDS 100% 10.800 7.560 N YPEL3 n/a
4 TRCN0000243053 GAGAACTGCAAGACCACTTTG pLKO_005 433 CDS 100% 10.800 7.560 N YPEL3 n/a
5 TRCN0000277324 AGGACCCAGCTCCTGTACATA pLKO_005 663 3UTR 100% 5.625 3.938 N Ypel3 n/a
6 TRCN0000191128 CAACCACATGATCAAAGACAA pLKO.1 522 CDS 100% 4.950 3.465 N Ypel3 n/a
7 TRCN0000277395 CAACCACATGATCAAAGACAA pLKO_005 522 CDS 100% 4.950 3.465 N Ypel3 n/a
8 TRCN0000200762 GAAATATGAGCAAGCCTTTGA pLKO.1 459 CDS 100% 4.950 3.465 N Ypel3 n/a
9 TRCN0000190584 GCTGGAAATATGAGCAAGCCT pLKO.1 455 CDS 100% 0.750 0.525 N Ypel3 n/a
10 TRCN0000190411 CATCCACTGTGAGAACTGCAA pLKO.1 423 CDS 100% 2.640 1.584 N Ypel3 n/a
11 TRCN0000277325 CATCCACTGTGAGAACTGCAA pLKO_005 423 CDS 100% 2.640 1.584 N Ypel3 n/a
12 TRCN0000190583 GCCTACCTCTTCAACTCTGTA pLKO.1 337 CDS 100% 4.950 2.475 Y Ypel3 n/a
13 TRCN0000277326 GCCTACCTCTTCAACTCTGTA pLKO_005 337 CDS 100% 4.950 2.475 Y Ypel3 n/a
14 TRCN0000135494 GCTGGAAATATGAGCAAGCTT pLKO.1 455 CDS 100% 3.000 2.100 N YPEL4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025347.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_16023 pDONR223 0% 95.5% 100% None (many diffs) n/a
2 ccsbBroad304_16023 pLX_304 0% 95.5% 100% V5 (many diffs) n/a
3 ccsbBroadEn_08123 pDONR223 100% 79.9% 87.3% None (many diffs) n/a
4 ccsbBroad304_08123 pLX_304 0% 79.9% 87.3% V5 (many diffs) n/a
5 TRCN0000472132 TTCAACAATTGAGCCTCATAACTA pLX_317 100% 79.9% 87.3% V5 (many diffs) n/a
6 ccsbBroadEn_05581 pDONR223 100% 79.6% 89% None (many diffs) n/a
7 ccsbBroad304_05581 pLX_304 0% 79.6% 89% V5 (many diffs) n/a
8 ccsbBroadEn_12753 pDONR223 100% 56.5% 59.2% None (many diffs) n/a
9 ccsbBroad304_12753 pLX_304 0% 56.5% 59.2% V5 (many diffs) n/a
10 TRCN0000472105 CCTAACTGAAGGGCCTCCGCGATT pLX_317 81.5% 56.5% 59.2% V5 (many diffs) n/a
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