Construct: shRNA TRCN0000278097

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009373.3-2202s21c1
DNA Barcode:
CCTGGTCTTTATCCTAAGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tgm2 (21817)
Gene Description:
transglutaminase 2, C polypeptide
Transcript:
RefSeq NM_009373.3 (CURRENT)
Match location:
Position 2202 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278097)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTCTTTATCCTAAGATA
Hairpin Sequence:
5'-CCGG-CCTGGTCTTTATCCTAAGATA-CTCGAG-TATCTTAGGATAAAGACCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTCTTTATCCTAAGATACTCGAGTATCTTAGGATAAAGACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTCTTTATCCTAAGATACTCGAGTATCTTAGGATAAAGACCAGG-3'

Other clones with same target sequence:

  1. TRCN0000009529