Construct: shRNA TRCN0000279037

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029478.3-952s21c1
DNA Barcode:
CCTTGTACCTTTCTGGACCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmp1 (75909)
Gene Description:
vacuole membrane protein 1
Transcript:
RefSeq NM_029478.3 (CURRENT)
Match location:
Position 952 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279037)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGTACCTTTCTGGACCTT
Hairpin Sequence:
5'-CCGG-CCTTGTACCTTTCTGGACCTT-CTCGAG-AAGGTCCAGAAAGGTACAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGTACCTTTCTGGACCTTCTCGAGAAGGTCCAGAAAGGTACAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGTACCTTTCTGGACCTTCTCGAGAAGGTCCAGAAAGGTACAAGG-3'

Other clones with same target sequence:

  1. TRCN0000173693