Construct: shRNA TRCN0000280150

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024459.2-600s21c1
DNA Barcode:
GTGGGCAACAATCTGAAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp3r1 (19058)
Gene Description:
protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)
Transcript:
RefSeq NM_024459.2 (CURRENT)
Match location:
Position 600 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280150)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGGCAACAATCTGAAAGAT
Hairpin Sequence:
5'-CCGG-GTGGGCAACAATCTGAAAGAT-CTCGAG-ATCTTTCAGATTGTTGCCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGGCAACAATCTGAAAGATCTCGAGATCTTTCAGATTGTTGCCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGGCAACAATCTGAAAGATCTCGAGATCTTTCAGATTGTTGCCCAC-3'

Other clones with same target sequence:

  1. TRCN0000012486