Construct: shRNA TRCN0000280638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024061.3-815s21c1
DNA Barcode:
CAAGTGCTTTCTCTGGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF655 (79027)
Gene Description:
zinc finger protein 655
Transcript:
RefSeq NM_024061.3 (CURRENT)
Match location:
Position 815 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGTGCTTTCTCTGGAGAAA
Hairpin Sequence:
5'-CCGG-CAAGTGCTTTCTCTGGAGAAA-CTCGAG-TTTCTCCAGAGAAAGCACTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGTGCTTTCTCTGGAGAAACTCGAGTTTCTCCAGAGAAAGCACTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGTGCTTTCTCTGGAGAAACTCGAGTTTCTCCAGAGAAAGCACTTG-3'

Other clones with same target sequence:

  1. TRCN0000016684