Construct: shRNA TRCN0000280879

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003359.2-548s21c1
DNA Barcode:
GCCATCAAAGAAGCTGATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UGDH (7358)
Gene Description:
UDP-glucose 6-dehydrogenase
Transcript:
RefSeq NM_003359.2 (NON-CURRENT)
Match location:
Position 548 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280879)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCAAAGAAGCTGATCTT
Hairpin Sequence:
5'-CCGG-GCCATCAAAGAAGCTGATCTT-CTCGAG-AAGATCAGCTTCTTTGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCAAAGAAGCTGATCTTCTCGAGAAGATCAGCTTCTTTGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCAAAGAAGCTGATCTTCTCGAGAAGATCAGCTTCTTTGATGGC-3'

Other clones with same target sequence:

  1. TRCN0000028112