Construct: shRNA TRCN0000028112
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_003359.1-310s1c1
- DNA Barcode:
- GCCATCAAAGAAGCTGATCTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- UGDH (7358)
- Gene Description:
- UDP-glucose 6-dehydrogenase
- Transcript:
- RefSeq NM_003359.1 (NON-CURRENT)
- Match location:
- Position 310 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000028112)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCATCAAAGAAGCTGATCTT
- Hairpin Sequence:
-
5'-CCGG-GCCATCAAAGAAGCTGATCTT-CTCGAG-AAGATCAGCTTCTTTGATGGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCATCAAAGAAGCTGATCTTCTCGAGAAGATCAGCTTCTTTGATGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCATCAAAGAAGCTGATCTTCTCGAGAAGATCAGCTTCTTTGATGGC-3'
Other clones with same target sequence:
-
TRCN0000280879
