Construct: shRNA TRCN0000281187

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014914.3-3605s21c1
DNA Barcode:
GCTGTTTAATAGAACGTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGAP1 (116987)
Gene Description:
ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Transcript:
RefSeq NM_014914.3 (NON-CURRENT)
Match location:
Position 3605 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281187)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTTAATAGAACGTGATT
Hairpin Sequence:
5'-CCGG-GCTGTTTAATAGAACGTGATT-CTCGAG-AATCACGTTCTATTAAACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTTAATAGAACGTGATTCTCGAGAATCACGTTCTATTAAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTTAATAGAACGTGATTCTCGAGAATCACGTTCTATTAAACAGC-3'

Other clones with same target sequence:

  1. TRCN0000106025
  2. TRCN0000158851
  3. TRCN0000316909