Construct: shRNA TRCN0000281225

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015691.3-2193s21c1
DNA Barcode:
GCTGGTGTTTAACGAAGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WWC3 (55841)
Gene Description:
WWC family member 3
Transcript:
RefSeq NM_015691.3 (CURRENT)
Match location:
Position 2193 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281225)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTGTTTAACGAAGTGTT
Hairpin Sequence:
5'-CCGG-GCTGGTGTTTAACGAAGTGTT-CTCGAG-AACACTTCGTTAAACACCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTGTTTAACGAAGTGTTCTCGAGAACACTTCGTTAAACACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTGTTTAACGAAGTGTTCTCGAGAACACTTCGTTAAACACCAGC-3'

Other clones with same target sequence:

  1. TRCN0000130435