Construct: shRNA TRCN0000281924

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001113735.1-207s21c1
DNA Barcode:
CTGGCAGACACACGAAGATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm13057 (100040861)
Gene Description:
predicted gene 13057
Transcript:
RefSeq NM_001113735.1 (CURRENT)
Match location:
Position 207 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281924)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGCAGACACACGAAGATAC
Hairpin Sequence:
5'-CCGG-CTGGCAGACACACGAAGATAC-CTCGAG-GTATCTTCGTGTGTCTGCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGCAGACACACGAAGATACCTCGAGGTATCTTCGTGTGTCTGCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGCAGACACACGAAGATACCTCGAGGTATCTTCGTGTGTCTGCCAG-3'

Other clones with same target sequence:

(none)