Construct: shRNA TRCN0000282418

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001101631.1-349s21c1
DNA Barcode:
TCTGCTGCACACCTGTCTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm3285 (100041351)
Gene Description:
predicted gene 3285
Transcript:
RefSeq NM_001101631.1 (CURRENT)
Match location:
Position 349 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282418)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGCTGCACACCTGTCTGTT
Hairpin Sequence:
5'-CCGG-TCTGCTGCACACCTGTCTGTT-CTCGAG-AACAGACAGGTGTGCAGCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGCTGCACACCTGTCTGTTCTCGAGAACAGACAGGTGTGCAGCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGCTGCACACCTGTCTGTTCTCGAGAACAGACAGGTGTGCAGCAGA-3'

Other clones with same target sequence:

(none)