Construct: shRNA TRCN0000283129

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183087.4-465s21c1
DNA Barcode:
GGCCTCTCTCGCTTGTGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam189a1 (70638)
Gene Description:
family with sequence similarity 189, member A1
Transcript:
RefSeq NM_183087.4 (CURRENT)
Match location:
Position 465 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000283129)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCCTCTCTCGCTTGTGATTA
Hairpin Sequence:
5'-CCGG-GGCCTCTCTCGCTTGTGATTA-CTCGAG-TAATCACAAGCGAGAGAGGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCCTCTCTCGCTTGTGATTACTCGAGTAATCACAAGCGAGAGAGGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCCTCTCTCGCTTGTGATTACTCGAGTAATCACAAGCGAGAGAGGCC-3'

Other clones with same target sequence:

(none)