Construct: shRNA TRCN0000283579

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001111141.1-1466s21c1
DNA Barcode:
TGGGACATCCGCAACCATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sprtn (244666)
Gene Description:
SprT-like N-terminal domain
Transcript:
RefSeq NM_001111141.1 (CURRENT)
Match location:
Position 1466 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000283579)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGACATCCGCAACCATATC
Hairpin Sequence:
5'-CCGG-TGGGACATCCGCAACCATATC-CTCGAG-GATATGGTTGCGGATGTCCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGACATCCGCAACCATATCCTCGAGGATATGGTTGCGGATGTCCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGACATCCGCAACCATATCCTCGAGGATATGGTTGCGGATGTCCCA-3'

Other clones with same target sequence:

(none)