Construct: shRNA TRCN0000285823

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016843.3-427s21c1
DNA Barcode:
GTTCTTCTCTTTCGTGAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atxn10 (54138)
Gene Description:
ataxin 10
Transcript:
RefSeq NM_016843.3 (CURRENT)
Match location:
Position 427 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285823)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCTTCTCTTTCGTGAACTT
Hairpin Sequence:
5'-CCGG-GTTCTTCTCTTTCGTGAACTT-CTCGAG-AAGTTCACGAAAGAGAAGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCTTCTCTTTCGTGAACTTCTCGAGAAGTTCACGAAAGAGAAGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCTTCTCTTTCGTGAACTTCTCGAGAAGTTCACGAAAGAGAAGAAC-3'

Other clones with same target sequence:

  1. TRCN0000183365