Construct: shRNA TRCN0000287028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001847.2-5102s21c1
DNA Barcode:
CCTGTGTCTGAAACGCTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL4A6 (1288)
Gene Description:
collagen type IV alpha 6 chain
Transcript:
RefSeq NM_001847.2 (NON-CURRENT)
Match location:
Position 5102 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGTCTGAAACGCTGAAA
Hairpin Sequence:
5'-CCGG-CCTGTGTCTGAAACGCTGAAA-CTCGAG-TTTCAGCGTTTCAGACACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGTCTGAAACGCTGAAACTCGAGTTTCAGCGTTTCAGACACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGTCTGAAACGCTGAAACTCGAGTTTCAGCGTTTCAGACACAGG-3'

Other clones with same target sequence:

  1. TRCN0000084136