Construct: shRNA TRCN0000287400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018822.3-1215s21c1
DNA Barcode:
CCCATCGACCAAGATTTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sgsh (27029)
Gene Description:
N-sulfoglucosamine sulfohydrolase (sulfamidase)
Transcript:
RefSeq NM_018822.3 (CURRENT)
Match location:
Position 1215 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCGACCAAGATTTCTAT
Hairpin Sequence:
5'-CCGG-CCCATCGACCAAGATTTCTAT-CTCGAG-ATAGAAATCTTGGTCGATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCGACCAAGATTTCTATCTCGAGATAGAAATCTTGGTCGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCGACCAAGATTTCTATCTCGAGATAGAAATCTTGGTCGATGGG-3'

Other clones with same target sequence:

  1. TRCN0000101842