Construct: shRNA TRCN0000287551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133932.2-761s21c1
DNA Barcode:
CCAGAAGATGAAGCTGAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tada3 (101206)
Gene Description:
transcriptional adaptor 3
Transcript:
RefSeq NM_133932.2 (CURRENT)
Match location:
Position 761 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAAGATGAAGCTGAACAT
Hairpin Sequence:
5'-CCGG-CCAGAAGATGAAGCTGAACAT-CTCGAG-ATGTTCAGCTTCATCTTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAAGATGAAGCTGAACATCTCGAGATGTTCAGCTTCATCTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAAGATGAAGCTGAACATCTCGAGATGTTCAGCTTCATCTTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000039289