Construct: shRNA TRCN0000287660

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025346.1-1300s21c1
DNA Barcode:
CACAAGGATGAGTTGCCGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rmnd5b (66089)
Gene Description:
required for meiotic nuclear division 5 homolog B
Transcript:
RefSeq NM_025346.1 (CURRENT)
Match location:
Position 1300 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287660)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAAGGATGAGTTGCCGATT
Hairpin Sequence:
5'-CCGG-CACAAGGATGAGTTGCCGATT-CTCGAG-AATCGGCAACTCATCCTTGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAAGGATGAGTTGCCGATTCTCGAGAATCGGCAACTCATCCTTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAAGGATGAGTTGCCGATTCTCGAGAATCGGCAACTCATCCTTGTG-3'

Other clones with same target sequence:

  1. TRCN0000039450