Construct: shRNA TRCN0000287842

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013733.3-333s21c1
DNA Barcode:
CGTAGGCTTGAGTACAAAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chaf1a (27221)
Gene Description:
chromatin assembly factor 1, subunit A (p150)
Transcript:
RefSeq NM_013733.3 (CURRENT)
Match location:
Position 333 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287842)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTAGGCTTGAGTACAAAGTT
Hairpin Sequence:
5'-CCGG-CGTAGGCTTGAGTACAAAGTT-CTCGAG-AACTTTGTACTCAAGCCTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTAGGCTTGAGTACAAAGTTCTCGAGAACTTTGTACTCAAGCCTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTAGGCTTGAGTACAAAGTTCTCGAGAACTTTGTACTCAAGCCTACG-3'

Other clones with same target sequence:

  1. TRCN0000109036