Construct: shRNA TRCN0000287953

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016906.4-453s21c1
DNA Barcode:
CCAGAAGTTGTTTGGAATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec61a1 (53421)
Gene Description:
Sec61 alpha 1 subunit (S. cerevisiae)
Transcript:
RefSeq NM_016906.4 (CURRENT)
Match location:
Position 453 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287953)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAAGTTGTTTGGAATGAT
Hairpin Sequence:
5'-CCGG-CCAGAAGTTGTTTGGAATGAT-CTCGAG-ATCATTCCAAACAACTTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAAGTTGTTTGGAATGATCTCGAGATCATTCCAAACAACTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAAGTTGTTTGGAATGATCTCGAGATCATTCCAAACAACTTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000111597