Construct: shRNA TRCN0000288046

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133969.2-804s21c1
DNA Barcode:
GCTCTGGATATAATCTGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp4v3 (102294)
Gene Description:
cytochrome P450, family 4, subfamily v, polypeptide 3
Transcript:
RefSeq NM_133969.2 (CURRENT)
Match location:
Position 804 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288046)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGGATATAATCTGTGAA
Hairpin Sequence:
5'-CCGG-GCTCTGGATATAATCTGTGAA-CTCGAG-TTCACAGATTATATCCAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGGATATAATCTGTGAACTCGAGTTCACAGATTATATCCAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGGATATAATCTGTGAACTCGAGTTCACAGATTATATCCAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000031637