Construct: shRNA TRCN0000288396

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007739.2-250s21c1
DNA Barcode:
GCTGGGAATACTGTTCATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col8a1 (12837)
Gene Description:
collagen, type VIII, alpha 1
Transcript:
RefSeq NM_007739.2 (CURRENT)
Match location:
Position 250 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288396)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGGAATACTGTTCATCAT
Hairpin Sequence:
5'-CCGG-GCTGGGAATACTGTTCATCAT-CTCGAG-ATGATGAACAGTATTCCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGGAATACTGTTCATCATCTCGAGATGATGAACAGTATTCCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGGAATACTGTTCATCATCTCGAGATGATGAACAGTATTCCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000091580