Construct: shRNA TRCN0000288675

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001924.2-5066s21c1
DNA Barcode:
GCCTAACACTATCCTGTAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTUS1 (57509)
Gene Description:
microtubule associated scaffold protein 1
Transcript:
RefSeq NM_001001924.2 (CURRENT)
Match location:
Position 5066 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288675)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAACACTATCCTGTAATT
Hairpin Sequence:
5'-CCGG-GCCTAACACTATCCTGTAATT-CTCGAG-AATTACAGGATAGTGTTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAACACTATCCTGTAATTCTCGAGAATTACAGGATAGTGTTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAACACTATCCTGTAATTCTCGAGAATTACAGGATAGTGTTAGGC-3'

Other clones with same target sequence:

  1. TRCN0000063673