Construct: shRNA TRCN0000288786

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005163.2-1091s21c1
DNA Barcode:
GATCCTCAAGAAGGAAGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKT1 (207)
Gene Description:
AKT serine/threonine kinase 1
Transcript:
RefSeq NM_005163.2 (CURRENT)
Match location:
Position 1091 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288786)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCTCAAGAAGGAAGTCAT
Hairpin Sequence:
5'-CCGG-GATCCTCAAGAAGGAAGTCAT-CTCGAG-ATGACTTCCTTCTTGAGGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCTCAAGAAGGAAGTCATCTCGAGATGACTTCCTTCTTGAGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCTCAAGAAGGAAGTCATCTCGAGATGACTTCCTTCTTGAGGATC-3'

Other clones with same target sequence:

  1. TRCN0000221553