Transcript: Human NM_005163.2

Homo sapiens AKT serine/threonine kinase 1 (AKT1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-25
Taxon:
Homo sapiens (human)
Gene:
AKT1 (207)
Length:
3008
CDS:
555..1997

Additional Resources:

NCBI RefSeq record:
NM_005163.2
NBCI Gene record:
AKT1 (207)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149266 TGTTGAGGGGAGCCTCACGT pXPR_003 TGG 143 10% 3 0.4883 AKT1 AKT1 75501
2 BRDN0001149414 TGTCATGGAGTACGCCAACG pXPR_003 GGG 691 48% 8 0.2326 AKT1 AKT1 75502
3 BRDN0001146935 GAAGGTGCGTTCGATGACAG pXPR_003 TGG 247 17% 4 0.0273 AKT1 AKT1 75500
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005163.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000221552 CGTGCCATGATCTGTATTTAA pLKO.1 2050 3UTR 100% 15.000 21.000 N AKT1 n/a
2 TRCN0000221555 CGCGTGACCATGAACGAGTTT pLKO.1 984 CDS 100% 4.950 6.930 N AKT1 n/a
3 TRCN0000288787 CGCGTGACCATGAACGAGTTT pLKO_005 984 CDS 100% 4.950 6.930 N AKT1 n/a
4 TRCN0000221554 GCATCGCTTCTTTGCCGGTAT pLKO.1 1766 CDS 100% 4.050 5.670 N AKT1 n/a
5 TRCN0000010162 GGACAAGGACGGGCACATTAA pLKO.1 1400 CDS 100% 13.200 9.240 N AKT1 n/a
6 TRCN0000295915 GGACAAGGACGGGCACATTAA pLKO_005 1400 CDS 100% 13.200 9.240 N AKT1 n/a
7 TRCN0000199454 GCAGCACGTGTACGAGAAGAA pLKO.1 1793 CDS 100% 4.950 3.465 N AKT1 n/a
8 TRCN0000010171 CTATGGCGCTGAGATTGTGTC pLKO.1 1310 CDS 100% 4.050 2.835 N AKT1 n/a
9 TRCN0000199889 GCACCTTCATTGGCTACAAGG pLKO.1 652 CDS 100% 4.050 2.835 N AKT1 n/a
10 TRCN0000010163 CGAGTTTGAGTACCTGAAGCT pLKO.1 998 CDS 100% 2.640 1.848 N AKT1 n/a
11 TRCN0000010174 GGACTACCTGCACTCGGAGAA pLKO.1 1337 CDS 100% 1.350 0.945 N AKT1 n/a
12 TRCN0000295914 GGACTACCTGCACTCGGAGAA pLKO_005 1337 CDS 100% 1.350 0.945 N AKT1 n/a
13 TRCN0000221553 GATCCTCAAGAAGGAAGTCAT pLKO.1 1091 CDS 100% 4.950 2.970 N AKT1 n/a
14 TRCN0000288786 GATCCTCAAGAAGGAAGTCAT pLKO_005 1091 CDS 100% 4.950 2.970 N AKT1 n/a
15 TRCN0000039795 GCCACGCTACTTCCTCCTCAA pLKO.1 623 CDS 100% 1.350 0.810 N AKT1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005163.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00046 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00046 pLX_304 43.5% 79.2% 57.1% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_14538 pDONR223 0% 100% 100% None n/a
4 ccsbBroad304_14538 pLX_304 35.1% 100% 100% V5 n/a
5 TRCN0000473539 CAATATTCCATCCCCTGATACTAT pLX_317 15.7% 100% 100% V5 n/a
6 TRCN0000491814 GCGATCTATGAGTGTCTGACCAGA pLX_317 25.1% 99.9% 100% V5 (not translated due to prior stop codon) 84C>A n/a
7 TRCN0000489962 CGCTTCAACGAGGCTGAGACCTTG pLX_317 24.7% 97.2% 96.9% V5 (many diffs) n/a
8 TRCN0000488923 CAAGAGACGTTTCTCGCTTCGGAA pLX_317 21.6% 96.9% 96.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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