Construct: shRNA TRCN0000290510

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138777.2-679s21c1
DNA Barcode:
GCACAGAGAAATGCTGGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRRF (92399)
Gene Description:
mitochondrial ribosome recycling factor
Transcript:
RefSeq NM_138777.2 (NON-CURRENT)
Match location:
Position 679 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290510)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGAGAAATGCTGGTGAA
Hairpin Sequence:
5'-CCGG-GCACAGAGAAATGCTGGTGAA-CTCGAG-TTCACCAGCATTTCTCTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGAGAAATGCTGGTGAACTCGAGTTCACCAGCATTTCTCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGAGAAATGCTGGTGAACTCGAGTTCACCAGCATTTCTCTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000045269