Construct: shRNA TRCN0000291861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006604.2-253s21c1
DNA Barcode:
CCCAAGCTGAAGAAGATTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RFPL3 (10738)
Gene Description:
ret finger protein like 3
Transcript:
RefSeq NM_006604.2 (CURRENT)
Match location:
Position 253 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGCTGAAGAAGATTCTA
Hairpin Sequence:
5'-CCGG-CCCAAGCTGAAGAAGATTCTA-CTCGAG-TAGAATCTTCTTCAGCTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGCTGAAGAAGATTCTACTCGAGTAGAATCTTCTTCAGCTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGCTGAAGAAGATTCTACTCGAGTAGAATCTTCTTCAGCTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000033756