Transcript: Human NM_006604.2

Homo sapiens ret finger protein like 3 (RFPL3), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
RFPL3 (10738)
Length:
1214
CDS:
19..885

Additional Resources:

NCBI RefSeq record:
NM_006604.2
NBCI Gene record:
RFPL3 (10738)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006604.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000033754 GCTCCTTCAATTCCACCTAAT pLKO.1 781 CDS 100% 10.800 6.480 N RFPL3 n/a
2 TRCN0000291859 GCTCCTTCAATTCCACCTAAT pLKO_005 781 CDS 100% 10.800 6.480 N RFPL3 n/a
3 TRCN0000033755 CCGCTGACTTTCCTCTTAGTA pLKO.1 625 CDS 100% 5.625 3.375 N RFPL3 n/a
4 TRCN0000291860 CCGCTGACTTTCCTCTTAGTA pLKO_005 625 CDS 100% 5.625 3.375 N RFPL3 n/a
5 TRCN0000033757 GCTTTGCTGTTGCTGTTCCAT pLKO.1 159 CDS 100% 3.000 1.800 N RFPL3 n/a
6 TRCN0000310091 GCTTTGCTGTTGCTGTTCCAT pLKO_005 159 CDS 100% 3.000 1.800 N RFPL3 n/a
7 TRCN0000033758 GCGGAAGTTCCAAGTGGATAT pLKO.1 291 CDS 100% 10.800 5.400 Y RFPL3 n/a
8 TRCN0000033756 CCCAAGCTGAAGAAGATTCTA pLKO.1 253 CDS 100% 5.625 2.813 Y RFPL3 n/a
9 TRCN0000291861 CCCAAGCTGAAGAAGATTCTA pLKO_005 253 CDS 100% 5.625 2.813 Y RFPL3 n/a
10 TRCN0000033689 GCCAACAACTTCCTCCTCATT pLKO.1 331 CDS 100% 4.950 2.475 Y RFPL1 n/a
11 TRCN0000033693 CGAGAGATTTGACGTGTCCAT pLKO.1 411 CDS 100% 2.640 1.320 Y RFPL1 n/a
12 TRCN0000033762 GTCTATACATTCAGGAGCGTA pLKO.1 730 CDS 100% 2.640 1.320 Y RFPL2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006604.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07672 pDONR223 100% 99.7% 99.6% None 241C>A;792A>G n/a
2 ccsbBroad304_07672 pLX_304 0% 99.7% 99.6% V5 241C>A;792A>G n/a
3 ccsbBroadEn_02517 pDONR223 100% 97.4% 94.4% None (many diffs) n/a
4 ccsbBroad304_02517 pLX_304 0% 97.4% 94.4% V5 (many diffs) n/a
5 TRCN0000481078 GTATTACCAGGTGCGATTAGCTGT pLX_317 51.6% 97.4% 94.4% V5 (many diffs) n/a
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