Construct: shRNA TRCN0000292693

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026124.3-768s21c1
DNA Barcode:
GCATTTCTGAATTTGTCCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1110008F13Rik (67388)
Gene Description:
RIKEN cDNA 1110008F13 gene
Transcript:
RefSeq NM_026124.3 (CURRENT)
Match location:
Position 768 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292693)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTTCTGAATTTGTCCATT
Hairpin Sequence:
5'-CCGG-GCATTTCTGAATTTGTCCATT-CTCGAG-AATGGACAAATTCAGAAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTTCTGAATTTGTCCATTCTCGAGAATGGACAAATTCAGAAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTTCTGAATTTGTCCATTCTCGAGAATGGACAAATTCAGAAATGC-3'

Other clones with same target sequence:

  1. TRCN0000174777