Construct: shRNA TRCN0000292897
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_023060.3-349s21c1
- DNA Barcode:
- GATTTGATGATGCTCGTGATT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Eefsec (65967)
- Gene Description:
- eukaryotic elongation factor, selenocysteine-tRNA-specific
- Transcript:
- RefSeq NM_023060.3 (CURRENT)
- Match location:
- Position 349 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000292897)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GATTTGATGATGCTCGTGATT
- Hairpin Sequence:
-
5'-CCGG-GATTTGATGATGCTCGTGATT-CTCGAG-AATCACGAGCATCATCAAATC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGATTTGATGATGCTCGTGATTCTCGAGAATCACGAGCATCATCAAATCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGATTTGATGATGCTCGTGATTCTCGAGAATCACGAGCATCATCAAATC-3'
Other clones with same target sequence:
-
TRCN0000193966
