Construct: shRNA TRCN0000293004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025551.3-363s21c1
DNA Barcode:
CTGGACAAACCATAAATTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndufa12 (66414)
Gene Description:
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12
Transcript:
RefSeq NM_025551.3 (CURRENT)
Match location:
Position 363 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGACAAACCATAAATTCAA
Hairpin Sequence:
5'-CCGG-CTGGACAAACCATAAATTCAA-CTCGAG-TTGAATTTATGGTTTGTCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGACAAACCATAAATTCAACTCGAGTTGAATTTATGGTTTGTCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGACAAACCATAAATTCAACTCGAGTTGAATTTATGGTTTGTCCAG-3'

Other clones with same target sequence:

  1. TRCN0000176776