Construct: shRNA TRCN0000293299

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004281.3-920s21c1
DNA Barcode:
ACATCTCCATTCCGGTGATAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BAG3 (9531)
Gene Description:
BAG cochaperone 3
Transcript:
RefSeq NM_004281.3 (CURRENT)
Match location:
Position 920 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293299)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATCTCCATTCCGGTGATAC
Hairpin Sequence:
5'-CCGG-ACATCTCCATTCCGGTGATAC-CTCGAG-GTATCACCGGAATGGAGATGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATCTCCATTCCGGTGATACCTCGAGGTATCACCGGAATGGAGATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATCTCCATTCCGGTGATACCTCGAGGTATCACCGGAATGGAGATGT-3'

Other clones with same target sequence:

(none)