Construct: shRNA TRCN0000294572

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133818.1-482s21c1
DNA Barcode:
CAAATTCCTCGTCGAGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
AI597479 (98404)
Gene Description:
expressed sequence AI597479
Transcript:
RefSeq NM_133818.1 (CURRENT)
Match location:
Position 482 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294572)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAATTCCTCGTCGAGGATTT
Hairpin Sequence:
5'-CCGG-CAAATTCCTCGTCGAGGATTT-CTCGAG-AAATCCTCGACGAGGAATTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAATTCCTCGTCGAGGATTTCTCGAGAAATCCTCGACGAGGAATTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAATTCCTCGTCGAGGATTTCTCGAGAAATCCTCGACGAGGAATTTG-3'

Other clones with same target sequence:

(none)